Chemical exchange saturation transfer (CEST) MRI provides images of molecular information and has recently been used for the detection of malignant brain tumors and the assessment of muscle tissue in cardiac infarction. Additionally, CEST has also been used to assess changes in a neurotransmitter -glutamate (Glu)- in both brain and spinal cord and has shown potential in a number of diseases including Alzheimer’s-like dementia, Parkinsonism and Huntington’s Disease and Motor neuron diseases.